TMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing

Area of Science:

• Proteomics
• Mass Spectrometry
• Biochemistry

Background:

• The TMTpro-16plex series expanded isobaric tagging reagents, building upon the TMT-11plex.
• The TMTpro molecule's proline backbone allows for additional isotope combinations.

Purpose of the Study:

• Introduce TMTpro-134C and TMTpro-135N labeling reagents for 18-plex global protein profiling.
• Demonstrate the utility of the TMTpro-18plex set for complex experimental designs.

Main Methods:

• Profiling the proteome and phosphoproteome of isogenic mammary epithelial cell lines using the TMTpro-18plex reagent set.
• Comparing the TMTpro-18plex data with a TMTpro-16plex experiment.

Main Results:

• Simultaneous global protein profiling of 18 samples with minimal missing values achieved.
• High quantitative correlation and similar depth of quantification observed between 18plex and 16plex experiments.
• Identified changes in protein abundance profiles across different conditions in isogenic cell lines.

Conclusions:

• TMTpro-18plex reagents significantly expand sample multiplexing capacity in mass spectrometry-based proteomics.
• Enables more complex and innovative experimental designs for proteomic and phosphoproteomic analyses.
• Facilitates in-depth investigation of biological conditions with increased sample numbers.