Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Multiple Comparison Tests

Multiple Comparison Tests

Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Using Distance Education to Teach the AAVMC's Spectrum of Care Subcompetencies: Positive Changes in Self-Efficacy as Self-Reported by Students Enrolled from Multiple Colleges of Veterinary Medicine in the United States.

Journal of veterinary medical education·2026

Same author

Clinical Features and Outcomes of Treatment for Effusive Feline Infectious Peritonitis with GS-441524 in Seventeen Retrovirus-Positive Cats.

Pathogens (Basel, Switzerland)·2026

Same author

Efficacy of Revolution<sup>®</sup> Plus (selamectin plus sarolaner) for the prevention of transmission of Borrelia burgdorferi from infected Ixodes scapularis to cats.

Parasites & vectors·2026

Same author

Nuclear Histone 3 Post-Translational Modification Profiling in Whole Cells using Spectral Flow Cytometry.

bioRxiv : the preprint server for biology·2026

Same author

Feline leukemia virus lifetime study: whole blood samples increase detection of low positive cats with extended long-term survival.

Journal of feline medicine and surgery·2025

Same author

Spectrum of care approach to animal shelter management of feline infectious peritonitis complicated by feline leukemia virus.

Frontiers in veterinary science·2025

Same journal

From farm to flask: stable genotypes and shifting microbiomes in the ecological dynamics of Balantioides coli from a One Health perspective.

Parasites & vectors·2026

Same journal

The American Heartworm Society and Association of Shelter Veterinarians' 2024 Shelter Heartworm Management Practices Survey.

Parasites & vectors·2026

Same journal

Evaluation of the diagnostic performance of a commercial ELISA based on two recombinant antigens for the diagnosis of Strongyloides stercoralis infection.

Parasites & vectors·2026

Same journal

Trouble from the tiger: first detection of dengue virus in Aedes albopictus in Australia during a 2024 dengue outbreak on Masig Island.

Parasites & vectors·2026

Same journal

Mitochondrial COI and 16S rDNA barcoding improve species delimitation of ixodid ticks in Peninsular Malaysia.

Parasites & vectors·2026

Same journal

Immune-reproductive cross talk in mosquitoes: molecular pathways and energy homeostasis.

Parasites & vectors·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 8, 2025

Author Spotlight: Coproparasitoscopic Examination of Dog Stools for Control and Prevention of Zoonotic Parasite Diseases

Author Spotlight: Coproparasitoscopic Examination of Dog Stools for Control and Prevention of Zoonotic Parasite Diseases

Published on: December 15, 2023

Optimizing heartworm diagnosis in dogs using multiple test combinations.

Jennifer N Lane¹, Annette Litster², Susan E Little³

¹Maddie's Shelter Medicine Program, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Gainesville, FL, 32610, USA.

Parasites & Vectors

|April 27, 2021

Summary

This summary is machine-generated.

Diagnosing heartworm (HW) in dogs is challenging, but combining antigen (Ag) and microfilariae (Mf) tests improves accuracy over single Ag tests. Further improvements are needed for rapid, inexpensive HW detection in shelters.

Keywords:

Antibody Antigen Diagnosis Heartworm Microfilaria

More Related Videos

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

Related Experiment Videos

Last Updated: Nov 8, 2025

Author Spotlight: Coproparasitoscopic Examination of Dog Stools for Control and Prevention of Zoonotic Parasite Diseases

Author Spotlight: Coproparasitoscopic Examination of Dog Stools for Control and Prevention of Zoonotic Parasite Diseases

Published on: December 15, 2023

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

Area of Science:

Veterinary Diagnostics
Parasitology
Infectious Diseases

Background:

Heartworm (HW) diagnostics utilize various methods targeting different Dirofilaria immitis life stages.
Antigen (Ag) and microfilariae (Mf) tests are common for dogs, while antibody (Ab) tests are validated for cats.
Heat-treatment (HT) can improve HW Ag test sensitivity by disrupting immune complexes.

Purpose of the Study:

To compare diagnostic methods for HW infection in high-risk dogs.
To evaluate individual and paired diagnostic tests, including feline Ab tests in dogs.
To assess the impact of heat-treatment on HW Ag test sensitivity.

Main Methods:

One hundred stray adult dogs in Florida shelters were tested.
Methods included microfilariae (Mf), HW Ag, and HW Ab tests (feline Ab tests used experimentally).
Two versions of each test platform were employed.

Main Results:

Point-of-care (POC) Ag tests identified 14 positive dogs; HT Ag testing identified an additional 12.
Individual Ag test sensitivity/specificity ranged from 50-57%/100% compared to HT Ag.
Pairing POC Ag with Mf tests improved sensitivity without sacrificing specificity.

Conclusions:

Screening dogs with both POC Ag and Mf tests enhances diagnostic performance over single Ag tests.
Current HW testing methods may miss over 25% of infected dogs.
Improved, accessible, and cost-effective HW testing solutions are needed for shelters.