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Related Experiment Videos

Changes in a nuclear matrix antigen during the cell cycle: interphase and mitotic cells.

I T Todorov1, P N Philipova, N Z Zhelev

  • 1Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.

Biology of the Cell
|January 1, 1988
PubMed
Summary

A 125 kDa nuclear matrix antigen (p125/6.5) shows increased levels and altered distribution during cell division. This protein is involved in late G2 phase and the G2/M transition, preparing cells for mitosis.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • A 125 kDa antigen (p125/6.5) is associated with the nuclear matrix.
  • This antigen is detected in proliferating human cells.

Purpose of the Study:

  • To investigate the behavior of the p125/6.5 antigen during the cell cycle in human cells.
  • To determine the role of the p125/6.5 protein in cell division.

Main Methods:

  • Indirect immunofluorescence using a specific monoclonal antibody.
  • Two-dimensional gel electrophoresis and immunoblotting.

Main Results:

  • During interphase, the p125/6.5 antigen exhibits a speckled nucleoplasmic distribution.
  • Mitosis shows increased antigen levels and diffuse nucleoplasmic localization, with negative staining on chromosomes.

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  • The antigen is present in the cytoplasm during anaphase and cytokinesis with decreasing intensity.

    • Conclusions:

    • The p125/6.5 protein accumulates during mitosis.
    • Evidence suggests the p125/6.5 protein plays a role in late G2 phase and the G2/M transition.
    • This protein is involved in preparing the cell for mitosis.