Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.4K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.4K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.3K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

5.8K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
5.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.8K
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

26.8K
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the...
26.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimating shear wave speeds and shear viscosities with a viscoelastic time-domain method.

The Journal of the Acoustical Society of America·2026
Same author

A wearable non-invasive sonogenetic pacemaker.

Nature biomedical engineering·2026
Same author

A programmable and self-adaptive ultrasonic wireless implant for personalized chronic pain management.

Nature electronics·2026
Same author

Shear wave elastography primer for the abdominal radiologist.

Abdominal radiology (New York)·2025
Same author

Breast ultrasound knobology and the knobology of twinkling for marker detection.

Translational breast cancer research : a journal focusing on translational research in breast cancer·2024
Same author

Robotic Ultrasound and Novel Collagen Analyses for Polycystic Kidney Disease Research Using Mice.

Kidney360·2024
Same journal

Concentric transmon qubit featuring fast tunability and an anisotropic magnetic dipole moment.

Applied physics letters·2026
Same journal

Wobulation using a tunable electrowetting prism applied to structured illumination microscopy.

Applied physics letters·2026
Same journal

Superconducting micro-resonator arrays with ideal frequency spacing.

Applied physics letters·2025
Same journal

Overlap junctions for high coherence superconducting qubits.

Applied physics letters·2025
Same journal

Controlling the thermal conductance of silicon nitride membranes at 100 mK temperatures with patterned metal features.

Applied physics letters·2025
Same journal

Overlap junctions for superconducting quantum electronics and amplifiers.

Applied physics letters·2025
See all related articles

Related Experiment Video

Updated: Nov 8, 2025

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.0K

Optical coherence viscometry.

Hsiao-Chuan Liu1, Matthew W Urban

  • 1Department of Radiology, Mayo Clinic, Rochester, Minnesota 55905, USA.

Applied Physics Letters
|April 28, 2021
PubMed
Summary
This summary is machine-generated.

Optical coherence viscometry (OCV) non-contactly measures fluid viscosity by analyzing capillary waves. This technique is sensitive and applicable to biological fluids, reducing sample volume requirements.

More Related Videos

Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment
07:02

Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment

Published on: June 30, 2023

1.8K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.2K

Related Experiment Videos

Last Updated: Nov 8, 2025

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.0K
Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment
07:02

Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment

Published on: June 30, 2023

1.8K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.2K

Area of Science:

  • Fluid mechanics
  • Biophysics
  • Optical metrology

Background:

  • Capillary waves are linked to fluid mechanical properties.
  • Measuring viscosity of small fluid volumes, like those in tissue cultures, presents challenges.
  • Overdamped effects can hinder capillary wave analysis in viscous fluids.

Purpose of the Study:

  • To introduce a noncontact technique, optical coherence viscometry (OCV), for measuring the viscosity of Newtonian fluids.
  • To demonstrate OCV's capability for analyzing capillary waves with reduced fluid quantities.
  • To validate OCV by comparing measurements with theoretical calculations.

Main Methods:

  • Generating capillary waves using transient acoustic radiation force to avoid overdamped effects.
  • Utilizing optical coherence tomography (OCT) for high-resolution, time-domain wave motion acquisition.
  • Applying Fourier methods to analyze wave velocity dispersion and attenuation relationships.

Main Results:

  • Successfully measured the viscosity of water, water-glycerol solutions, and biological plasma.
  • Demonstrated OCV's sensitivity to wave perturbations.
  • Validated experimental results against theoretical calculations.

Conclusions:

  • Optical coherence viscometry (OCV) is a viable noncontact method for viscosity measurement.
  • The technique significantly reduces the required fluid volume, making it suitable for biological samples.
  • OCV shows promise for applications in cell biology and tissue engineering, particularly for lipid membrane analysis.