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Array-Based N-Glycan Profiling of Cells in Culture
Peggi M Angel1, Anand S Mehta2, Richard R Drake2
1Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA. angelp@musc.edu.
Methods in Molecular Biology (Clifton, N.J.)
|April 28, 2021
Summary
This study introduces a new method for analyzing N-glycans in cells using imaging mass spectrometry. This technique allows researchers to study N-glycan changes in cell cultures, complementing tissue analysis.
Area of Science:
- Glycomics
- Mass Spectrometry
- Cell Biology
Background:
- N-glycan imaging mass spectrometry (N-glycan IMS) is crucial for analyzing N-glycan localization in tissues.
- Studying disease-associated N-glycan signatures in cell cultures has been challenging.
Purpose of the Study:
- To adapt N-glycan IMS workflows for cells cultured in an array format.
- To enable detailed analysis of N-glycan profiles in cellular models.
Main Methods:
- Cells were cultured on glass slides in an array format.
- Cells were fixed to preserve morphology.
- PNGase F enzyme was used to release N-glycans for analysis.
- N-glycans were profiled using imaging mass spectrometry.
Main Results:
- A protocol was successfully developed to adapt tissue N-glycan IMS to cell arrays.
- This method allows for the imaging and characterization of N-glycans in cellular samples.
- The protocol facilitates the study of N-glycan signatures in a controlled cellular environment.
Conclusions:
- The described protocol enables the application of N-glycan IMS to cell cultures.
- This advancement provides a valuable tool for investigating N-glycan regulation and disease association in cellular models.
- This method bridges the gap between tissue analysis and cellular studies in glycomics.
