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Indicators02:39

Indicators

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Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are...
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Methods for quantifying phenolphthalein in slimming tea.

Steven Suryoprabowo1, Xinxin Xu1, Hua Kuang1

  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. murel@163.com and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

Journal of Materials Chemistry. B
|April 28, 2021
PubMed
Summary
This summary is machine-generated.

Online slimming teas often contain hazardous phenolphthalein. Researchers developed rapid immunochromatography gold-labeled monoclonal antibody (IGM) and fluorescence-labeled monoclonal antibody (IFM) methods to detect it, with IFM showing higher sensitivity.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Food Safety

Background:

  • Rising obesity rates and social media have increased online sales of slimming beverages.
  • These products are frequently adulterated with undeclared hazardous pharmaceutical compounds, such as phenolphthalein.
  • Ensuring the safety of weight-loss products requires reliable detection methods for contaminants.

Purpose of the Study:

  • To develop and validate rapid and sensitive immunoassay methods for detecting phenolphthalein in slimming beverages.
  • To compare the performance of immunochromatography gold-labeled monoclonal antibody (IGM) and fluorescence-labeled monoclonal antibody (IFM) techniques.
  • To provide a tool for quality control of weight-loss products.

Main Methods:

  • Development of monoclonal antibodies (mAbs) and coating antigens in-house.
  • Establishment of immunochromatography gold-labeled mAb (IGM) and fluorescence-labeled mAb (IFM) assays.
  • Testing and optimization of methods using phosphate-buffered saline (PBS) and slimming tea samples.

Main Results:

  • Optimal cut-off limits were determined as 250 ng/mL for IFM and 500 ng/mL for IGM.
  • Mean recoveries ranged from 96.2% to 104.7% (IGM) and 90.7% to 104.7% (IFM).
  • The IFM method demonstrated superior sensitivity and provided results within 10 minutes.

Conclusions:

  • The developed IFM and IGM methods are rapid, sensitive, and convenient for detecting phenolphthalein.
  • The IFM assay offers enhanced sensitivity compared to the IGM assay.
  • These novel techniques can be effectively applied for the quality control and safety assessment of slimming beverages.