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Updated: Nov 7, 2025

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

[New technologies for renal pathology: Transcriptomics on paraffin-embedded fixed tissue].

Blaise Robin¹, Jessy Dagobert¹, Pierre Isnard²

¹Paris Translational Research Center for Organ Transplantation, 56, rue Leblanc, 75015 Paris, France; Université de Paris, 56, rue Leblanc, 75015 Paris, France; Inserm U970, 56, rue Leblanc, 75015 Paris, France.

Nephrologie & Therapeutique

|April 29, 2021

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New high-throughput transcriptomics technologies are changing nephrology. The NanoString nCounter system offers a complementary approach, analyzing formalin-fixed samples for potential clinical use.

Area of Science:

Nephrology
Molecular Biology
Biotechnology

Background:

High-throughput genomics and transcriptomics have advanced clinical nephrology.
Existing methods include microarrays, next-generation sequencing (NGS), and reverse transcriptase quantitative PCR (RT-qPCR).

Purpose of the Study:

To introduce the NanoString nCounter system as a novel transcriptomic analysis tool.
To evaluate its potential for routine clinical application in nephrology.

Main Methods:

The study focuses on the nCounter system's capabilities.
It highlights its ability to analyze formalin-fixed paraffin-embedded (FFPE) samples.

Main Results:

The nCounter system provides a complementary approach to existing transcriptomic technologies.

Keywords:

Kidney transplantation Molecular pathology Nephrology Néphrologie Pathologie moléculaire Transcriptomic Transcriptomique Transplantation rénale

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Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

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Related Experiment Videos

Last Updated: Nov 7, 2025

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

It is capable of analyzing FFPE samples, which are common in clinical settings.

Conclusions:

The NanoString nCounter system is a viable candidate for integrating transcriptomics into routine nephrology practice.
Its ability to work with FFPE samples makes it practical for clinical implementation.