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Modeling and Evaluation of Murine Diabetic Cardiomyopathy Model
Published on: November 29, 2024
973
Hyperglycemia and hyperlipidemia can induce morphophysiological changes in rat cardiac cell line
Rocío Varela1, Inés Rauschert1,2, Gerardo Romanelli1
1Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia, 3318, CP, 11600, Montevideo, Uruguay.
Biochemistry and Biophysics Reports
|April 29, 2021
Summary
High glucose and palmitic acid media significantly increased cell death and altered morphology in H9c2 cardiac cells. These changes, linked to actin cytoskeleton reordering and increased stiffness, suggest a role for gluco- and lipotoxicity in diabetic cardiomyopathy.
Area of Science:
- Cardiovascular Biology
- Cellular Physiology
- Diabetic Complications
Background:
- Diabetic cardiomyopathy is a significant complication of diabetes mellitus.
- Hyperglycemia and hyperlipidemia are key metabolic derangements in diabetes.
- Understanding the cellular mechanisms underlying diabetic cardiomyopathy is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the effects of combined hyperglycemic and hyperlipidemic conditions on H9c2 cardiac cells.
- To quantify cell death, apoptosis, and morphological changes under these conditions.
- To explore the relationship between actin cytoskeleton reordering, cell stiffness, and these cellular alterations.
Main Methods:
- H9c2 cardiac cells were cultured under control, hyperglycemic, and hyperlipidemic conditions.
- Hyperglycemic and hyperlipidemic conditions (HHC) were established using high glucose and palmitic acid.
- Cell death, apoptosis, cell morphology, actin cytoskeleton organization, and cell stiffness (Young's modulus) were quantified.
Main Results:
- HHC significantly increased total cell death by 5.2-fold compared to control.
- Apoptotic cell death constituted 38.6% late and 8.3% early apoptosis under HHC.
- HHC induced significant changes in cell morphology, actin cytoskeleton reordering, and increased cell stiffness, particularly in the peripheral zone.
Conclusions:
- Combined hyperglycemia and hyperlipidemia induce significant cell death and morphophysiological changes in cardiac cells.
- Reordering of the actin cytoskeleton correlates with increased cell stiffness under HHC.
- These findings support the central role of gluco- and lipotoxicity in the pathogenesis of diabetic cardiomyopathy.
