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Demonstration of N-acetylchondrosine-degrading beta-glucuronidase in rabbit liver
T Nakamura1, K Takagaki, M Majima
1Department of Biochemistry, Hirosaki University School of Medicine, Japan.
FEBS Letters
|July 4, 1988
Abstract:
N-Acetylchondrosine was incubated at pH 4.0 with a rabbit-liver crude enzyme extract. Gel filtration of the reaction products on Sephadex G-15 revealed the presence of monosaccharide liberated from the disaccharide. The monosaccharide fraction was analyzed by gas-liquid chromatography, and identified as a mixture of glucuronic acid and N-acetylgalactosamine. These results indicate the presence of beta-glucuronidase, which degrades N-acetylchondrosine, in rabbit liver. The discovery of the presence of this enzyme may help to establish the complete degradation process of chondroitin sulfates.