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Updated: Nov 7, 2025

Functional Calcium Imaging in Developing Cortical Networks

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

Analyzing Developing Brain-On-Chip Cultures with the CALIMA Calcium Imaging Tool.

Elles A L Raaijmakers¹, Nikki Wanders², Rob M C Mestrom¹

¹Electromagnetics Group, Department of Electrical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands.

|April 30, 2021

Summary

CALIMA 2.0 analyzes calcium imaging data from brain-on-chip models. This software tool helps researchers study neural network formation and brain cell activity in real-time.

Keywords:

brain-on-chip culture calcium fluorescence imaging neuronal network software tool

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Published on: February 18, 2020

Area of Science:

Neuroscience
Bioengineering
Computational Biology

Background:

Brain-on-chip (BoC) models replicate the brain's microenvironment for studying neural (patho)physiology and drug responses.
Advances in BoC technology involve microfabrication and computer-steered feedback to control neuronal activity.
Calcium imaging (CI) is a common technique for visualizing neuronal activity in these models.

Purpose of the Study:

To introduce CALIMA 2.0, an updated software for analyzing calcium imaging data from BoC cultures.
To evaluate CALIMA 2.0's capability in analyzing large CI datasets and identifying network formation.
To assess the processing speed of CALIMA 2.0 for real-time data analysis.

Main Methods:

Utilized three previously published datasets of calcium imaging data from BoC cultures.
Applied CALIMA 2.0 software to detect and analyze fluorescent signals.
Correlated cellular activity to identify network formation and assess culture maturity.
Measured the processing speed of CALIMA 2.0 for various data acquisition rates.

Main Results:

CALIMA 2.0 successfully analyzed large CI datasets, interpreting cellular activity in BoC cultures.
The software demonstrated its utility in studying the activity and maturity of BoC models.
Processing speed analysis confirmed CALIMA 2.0 can handle data acquisition rates up to 5 Hz in real-time on standard hardware.

Conclusions:

CALIMA 2.0 is an effective tool for analyzing calcium imaging data in brain-on-chip research.
The software facilitates the study of neural network formation and brain cell activity.
CALIMA 2.0 offers efficient real-time data processing, supporting advanced neuroscience research.