Related Experiment Video
Updated: Nov 7, 2025
18:57
Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
Published on: October 17, 2013
46.8K
Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration
Vittorio Abruzzese1, Ilenia Matera1, Fabio Martinelli1
1Department of Sciences, University of Basilicata, 85100 Potenza, Italy.
International Journal of Molecular Sciences
|April 30, 2021
Summary
Quercetin, a dietary flavonoid, influences ABCC6 transporter expression in liver cells. This study suggests quercetin may mitigate oxidative stress responses impacting cell motility and cytoskeleton dynamics.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Quercetin, a flavonoid, exhibits antioxidant, anti-inflammatory, and anti-cancer properties.
- Quercetin modulates multidrug-resistance transporters and ecto-5'-nucleotidase (NT5E/CD73), impacting tumor progression.
- ABCC6 transporter mutations cause pseudoxanthoma elasticum; ABCC6 influences cell motility and cytoskeleton via purinergic signaling.
Purpose of the Study:
- Investigate Quercetin's effect on ABCC6 transporter expression in HepG2 cells.
- Explore the role of ABCC6 in cellular responses to oxidative stress.
- Determine if Quercetin mitigates oxidative stress-induced changes in ABCC6 expression and cell motility.
Main Methods:
- Quantitative Reverse-Transcription PCR (RT-qPCR) for gene expression.
- Western blot for protein expression analysis.
- Laser confocal microscopy and wound-healing assays for cell motility and cytoskeleton dynamics.
Main Results:
- Quercetin's impact on ABCC6 gene and protein expression in HepG2 cells was evaluated.
- Actin cytoskeleton dynamics and cell motility were analyzed under Quercetin treatment.
- The study explored the potential involvement of the AKT pathway in ABCC6 regulation.
Conclusions:
- Quercetin influences ABCC6 expression and cellular processes in HepG2 cells.
- ABCC6 expression may be regulated by the AKT pathway in response to oxidative stress.
- Quercetin-like flavonoids show potential in mitigating oxidative stress-related cellular adaptations.
More Related Videos
Related Concept Videos
01:31ABC Transporters: Exporter
5.6K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
5.6K
01:06Carrier-Mediated Transport
725
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
725
01:27ABC Transporters: Importer
3.1K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
3.1K
01:14Hepatic Drug Clearance: Role of Transporters
155
In the liver and bile canaliculi, influx and efflux transporters modification can influence intrinsic clearance. Transporters play a significant role in moving drugs within liver cells. Elaborate models, such as the Biopharmaceutical Classification System (BCS), are essential to relate transporters to drug disposition. This system categorizes drugs into four classes based on solubility and permeability, providing insights into elimination routes and the effects of transporters following oral...
155
01:23Transcellular Transport of Solutes
4.2K
Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
4.2K
01:07Physiological Pharmacokinetic Models: Incorporating Hepatic Transporter-Mediated Clearance
129
Drug transporters are critical in drug absorption, distribution, and excretion processes. They should be included in physiological-based pharmacokinetic (PBPK) models, which help predict human drug disposition. However, predicting this is challenging during drug development, especially when liver transport is involved. However, with a realistic representation of body transport processes, an accurate model may be possible.
A recent model describes pravastatin's hepatobiliary excretion,...
A recent model describes pravastatin's hepatobiliary excretion,...
129