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Related Experiment Video

Updated: Nov 7, 2025

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting.

Alex A Zhukov¹, Robyn H Pritchard², Mick J Withers²

¹Cellular Highways Ltd., Melbourn Science Park, Melbourn, Cambridgeshire SG8 6EE, UK.

|April 30, 2021

Summary

This summary is machine-generated.

We developed a parallel vortex-actuated cell sorter (VACS) for extremely high-throughput microfluidic cell sorting. This new system sorts particles 16 times faster than single-stream VACS and 10 times faster than commercial sorters.

Keywords:

VACS cell sorting cytometry high-throughput microfluidics

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Area of Science:

Biotechnology
Microfluidics
Cell Sorting

Background:

Conventional cell sorting methods face throughput limitations.
Vortex-actuated cell sorter (VACS) offers a promising approach for cell manipulation.
Scaling VACS technology is crucial for processing large cell populations.

Purpose of the Study:

To develop and demonstrate an ultra-high-throughput microfluidic cell sorting system.
To enhance the sorting rate of VACS technology through parallelization.
To enable processing of significantly larger cell numbers for various applications.

Main Methods:

Designed and fabricated a parallel microfluidic sorter chip.
Integrated parallel cytometry instrumentation, including optics, electronics, and control software.
Implemented a parallelized vortex-actuated cell sorting strategy.

Main Results:

Achieved sorting rates 16 times higher than single-stream VACS devices.
Demonstrated sorting speeds approximately 10 times faster than commercial cell sorters for equivalent procedures.
Successfully sorted lymphocyte-sized particles at unprecedented throughput.

Conclusions:

Parallelization of VACS technology significantly boosts microfluidic cell sorting throughput.
This advancement has the potential to overcome current limitations in processing large cell numbers.
The developed system opens new possibilities for applications requiring high-volume cell processing.