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Development of the Heart

Development of the Heart

The development of the human heart, a crucial organ, commences from the mesoderm on the 18th or 19th day after fertilization. This process initiates in the cardiogenic area, a group of mesodermal cells at the embryo's head end, which evolves into elongated strands known as cardiogenic cords. These cords undergo a transformation to form hollow-centered endocardial tubes.
As the embryo undergoes lateral folding, these paired tubes approach each other, merging into a single primitive heart...

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Related Experiment Video

Updated: Nov 7, 2025

Recovery of Adult Zebrafish Hearts for High-throughput Applications

Recovery of Adult Zebrafish Hearts for High-throughput Applications

Published on: December 12, 2014

Persistent Ventricle Partitioning in the Adult Zebrafish Heart.

Catherine Pfefferli¹, Hannah R Moran², Anastasia Felker²

¹Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland.

Journal of Cardiovascular Development and Disease

|April 30, 2021

Summary

The study tracks first heart field (FHF) cells in zebrafish, revealing their lasting presence in adult ventricles. This demonstrates ongoing cell-based compartmentalization in the zebrafish heart without physical septation.

Keywords:

cardiac ventricle first heart field heart development lineage tracing zebrafish

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Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Published on: February 9, 2022

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Last Updated: Nov 7, 2025

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Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

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Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Published on: February 9, 2022

Area of Science:

Developmental Biology
Cardiovascular Research
Zebrafish Models

Background:

Vertebrate heart development involves integrating cells from the first heart field (FHF) and second heart field (SHF).
In mammals, FHF and SHF contributions are crucial for forming distinct heart chambers and septation.
Zebrafish, with a single ventricle, also integrate FHF and SHF lineages, but their adult contributions are not fully understood.

Purpose of the Study:

To characterize the myocardial contribution of first heart field (FHF) descendants in the developing and adult zebrafish ventricle.
To investigate the persistence and spatial distribution of FHF-derived cardiomyocytes in the adult zebrafish heart.

Main Methods:

Utilized late gastrulation-stage labeling in zebrafish embryos.
Employed drl-driven CreERT2 recombinase and a myocardium-specific, myl7-controlled, loxP reporter system for lineage tracing.
Examined FHF cardiomyocyte distribution in both embryonic and adult zebrafish hearts.

Main Results:

Late gastrulation labeling predominantly marked FHF-derived cells in the outer curvature and right side of the embryonic ventricle.
Lineage-labeled hearts in adult zebrafish retained significant FHF cardiomyocyte populations.
These FHF cardiomyocytes were located in a ventricular region opposite the atrium, including the apex.

Conclusions:

The study confirms the persistence of FHF-derived cardiomyocytes in the adult zebrafish ventricle.
Findings support the concept of cell-based compartmentalization within the adult zebrafish ventricle, even without physical septation.
Adds to the understanding of myocardial lineage contributions in non-mammalian vertebrate heart development.