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Updated: Nov 7, 2025

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
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Comparative Analysis of CTRP-Mediated Effects on Cardiomyocyte Glucose Metabolism: Cross Talk between AMPK and Akt

Ling Li1, Muhammad Aslam2, Benedikt H Siegler1

  • 1Institute of Physiology, Justus Liebig University Giessen, 35392 Giessen, Germany.

Cells
|April 30, 2021
PubMed

Insights

C1q/tumor necrosis factor-alpha-related proteins (CTRPs) influence heart cell metabolism. Specific CTRPs enhance glucose uptake via the AMPK pathway, offering potential therapeutic strategies for glucose homeostasis.

Area of Science:

  • Cardiovascular Biology
  • Metabolic Regulation
  • Cell Signaling

Background:

  • C1q/tumor necrosis factor-alpha-related proteins (CTRPs) are known for cardiovascular protection.
  • Their specific roles in cardiomyocyte glucose and fatty acid metabolism remain largely unexplored.

Purpose of the Study:

  • To investigate the effects of various CTRPs on glucose and fatty acid metabolism in cardiomyocytes.
  • To elucidate the involvement of AMP-activated protein kinase (AMPK) and Akt signaling pathways.

Main Methods:

  • Primary adult rat cardiomyocytes and H9C2 cardiomyoblasts were treated with recombinant CTRPs.
  • Glucose and fatty acid uptake, gene expression related to metabolism, and signaling pathway activation (AMPK, Akt) were assessed.
  • The role of adiponectin receptor 1 was examined.

Main Results:

  • CTRP2, 7, 9, and 13 significantly increased glucose uptake and GLUT1/GLUT4 translocation in cardiomyocytes.
  • These CTRPs modulated the expression of key metabolic enzymes.
  • AMPK activation was upstream of Akt activation in CTRP signaling.
  • Adiponectin receptor 1 partially mediated CTRP effects on glucose metabolism.

Conclusions:

  • Specific CTRPs (CTRP2, 7, 9, 13) enhance cardiomyocyte glucose uptake through AMPK-dependent signaling.
  • Targeting the AMPK pathway with CTRPs presents a potential therapeutic approach for improving glucose homeostasis.

Keywords:
AMPKAdipoRAktC1q familyadiponectinglucose metabolism

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