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Related Experiment Video

Updated: Nov 7, 2025

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

415

TP63 Is Significantly Upregulated in Diabetic Kidney.

Sitai Liang1, Bijaya K Nayak1, Kristine S Vogel1

  • 1Department of Cell Systems and Anatomy, The University of Texas Health Science Center, San Antonio, TX 78229, USA.

International Journal of Molecular Sciences
|April 30, 2021
PubMed
Summary

Tumor protein 63 (TP63) is upregulated in diabetic mouse kidneys and kidney cells under high glucose. Downregulating TP63 increases insulin receptor substrate 1 (IRS-1) and glucose uptake, suggesting TP63 as a therapeutic target for diabetic kidney disease.

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Area of Science:

  • Nephrology
  • Endocrinology
  • Molecular Biology

Background:

  • The role of tumor protein 63 (TP63) in diabetes-related kidney complications is unknown.
  • Diabetic nephropathy is a major complication of diabetes mellitus.

Purpose of the Study:

  • To investigate the role of TP63 in regulating insulin receptor substrate 1 (IRS-1) and downstream signaling in diabetic kidney disease.
  • To explore TP63 as a potential therapeutic target for diabetic nephropathy.

Main Methods:

  • RNA sequencing of kidneys from diabetic (db/db) and wild-type mice.
  • In vitro studies using mouse proximal tubular cells exposed to high glucose (HG) and normal glucose (NG).
  • TP63 downregulation using siRNA, AMPK activation with AICAR, and tuberin overexpression.
Keywords:
IRS-1TP63diabeteskidney

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Related Experiment Videos

Last Updated: Nov 7, 2025

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

415
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.1K
Whole-Kidney Three-Dimensional Staining with CUBIC
04:31

Whole-Kidney Three-Dimensional Staining with CUBIC

Published on: July 18, 2022

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Main Results:

  • TP63 expression was significantly increased in kidneys of diabetic mice and HG-exposed cells.
  • TP63 downregulation led to increased IRS-1 expression and AMPK activation.
  • TP63 knockdown enhanced glucose uptake, while IRS-1 knockdown reduced it.

Conclusions:

  • TP63 plays a role in regulating IRS-1 expression and glucose uptake in the context of diabetes.
  • TP63 is a potential therapeutic target for preventing kidney complications in diabetes.