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A rapid method for processing very large numbers of tissue sections for immunohistochemical hybridoma screening
1Department of Biology, University of Oregon, Eugene 97403.
Journal of Immunological Methods
|June 28, 1988
Abstract:
A hybridoma screening format which facilitates the processing of thousands of tissue sections for immunohistochemical analysis is described. The method utilizes sterile replica transfers of monoclonal antibody-containing test supernatants from 96-well culture plates to tissue sections mounted in appropriately sized 8 X 12 arrays, and is extremely rapid and inexpensive.