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Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

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Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
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Preparation of Amides01:29

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Amides are synthesized by treating carboxylic acids with amines in the presence of dehydrating agents like dicyclohexylcarbodiimide (DCC).
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Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources.

Ruidong Ni1, Suzeeta Bhandari1, Perry R Mitchell1

  • 1Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA.

Molecules (Basel, Switzerland)
|April 30, 2021
PubMed
Summary

This study details chemical synthesis and characterization methods for fatty acid amides. These methods enable the analysis of fatty acid amidomes across various biological systems, including insects and mammals.

Keywords:
ApisBombyxDrosophilaN18TG2Triboliumanandamidechoroid plexusfatty acid amideliquid chromatography/quadrupole time-of-flight mass spectrometry

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Area of Science:

  • Lipidomics
  • Biochemistry
  • Chemical Synthesis

Background:

  • Fatty acid amides are a diverse and underappreciated class of biologically occurring lipids.
  • Understanding their roles requires robust analytical methods for their detection and quantification in biological systems.

Purpose of the Study:

  • To describe methods for the chemical synthesis and characterization of specific fatty acid amides.
  • To establish standards for the analysis of fatty acid amidomes.
  • To present fatty acid amidomes from diverse biological sources.

Main Methods:

  • Chemical synthesis of targeted fatty acid amides.
  • Characterization of synthesized fatty acid amides using analytical techniques.
  • Utilizing synthesized and commercial standards for fatty acid amidome analysis.

Main Results:

  • Successful synthesis and characterization of specific fatty acid amides.
  • Established standards for fatty acid amidome quantification.
  • Presented fatty acid amidomes from mouse cells, sheep cells, and multiple insect species (Drosophila melanogaster, Bombyx mori, Apis mellifera, Tribolium castaneum).

Conclusions:

  • The developed methods provide a foundation for comprehensive fatty acid amidome research.
  • This work facilitates the study of fatty acid amide roles in various biological contexts.
  • Comparative analysis of amidomes across species highlights potential evolutionary or functional differences.