Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Female Sex is Associated with Adverse Cardiac Remodeling and Increased Antihypertensive Requirements After Thoracic Endovascular Aortic Repair.

Annals of vascular surgery·2026
Same author

Bioorthogonal "Click Chemistry" Bone Cement Enables Pro-Translational Spinal Fusion in a Large Animal Sheep Model.

ACS biomaterials science & engineering·2026
Same author

Case 28-2025: A Man with Abdominal Pain, Fever, and Hypoxemia.

The New England journal of medicine·2026
Same author

Navigating Complexities in the Liver Intensive Care Unit: Bridging Critical Care and Hepatology.

The American journal of gastroenterology·2026
Same author

Wearable Sleep Measures May Improve Machine Learning Prediction of Home-based Pulmonary Rehabilitation Engagement Among Patients With Chronic Obstructive Pulmonary Disease: A Proof-of-Concept Study.

medRxiv : the preprint server for health sciences·2025
Same author

Development of a bioactive, piezoelectric PVDF-TrFE scaffold with evaluation of tissue reaction for potential in nerve repair.

Biomaterials science·2025
Same journal

Precision Proteomic Profiling of Systemic Lupus Erythematosus-Correlating Disease Activity and Complement Levels with Clinical Phenotypes.

Biomedicines·2026
Same journal

The Role of Salivary Microbiota in Pancreatic Cancer: From Screening to Tumor Progression and Treatment Response.

Biomedicines·2026
Same journal

Diagnostic Utility of Surface Electromyography for Identifying Muscles Affected by Myofascial Trigger Points: A Scoping Review.

Biomedicines·2026
Same journal

Performance Assessment of a Locally Semi-Automated NGS-Based Workflow for Homologous Recombination Deficiency Testing in High-Grade Serous Ovarian Carcinoma.

Biomedicines·2026
Same journal

Coupling and Uncoupling Pleiotropy Between Hypertension and Type 2 Diabetes Contribute to Exploring Potential Heterogeneity in Cardiovascular Risk in East Asian Population.

Biomedicines·2026
Same journal

Maternal Response to Therapeutic Plasma Exchange in Early Gestation: A Case Series of Thrombotic Microangiopathies and Neurological Disorders.

Biomedicines·2026
See all related articles

Related Experiment Video

Updated: Nov 7, 2025

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration
08:52

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

14.6K

Promoting Neuronal Outgrowth Using Ridged Scaffolds Coated with Extracellular Matrix Proteins.

Ahad M Siddiqui1, Rosa Brunner2, Gregory M Harris3

  • 1Department of Neurology, Mayo Clinic, Rochester, MN 55905, USA.

Biomedicines
|April 30, 2021
PubMed
Summary
This summary is machine-generated.

A new biomaterial scaffold using oligo(poly(ethylene glycol)fumarate) (OPF) promotes nerve regeneration after spinal cord injury (SCI). This scaffold supports cell attachment, guides axon growth, and aids functional recovery.

Keywords:
Schwann cellsaxonal regenerationcell attachmentextracellular matrixneuronal outgrowthscaffoldsspinal cord

More Related Videos

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.4K
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
09:29

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Published on: August 16, 2014

12.5K

Related Experiment Videos

Last Updated: Nov 7, 2025

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration
08:52

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

14.6K
Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.4K
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
09:29

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Published on: August 16, 2014

12.5K

Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Regenerative Medicine

Background:

  • Spinal cord injury (SCI) causes irreversible damage, including cell death and axonal loss, with limited regenerative capacity.
  • Current therapies for SCI are insufficient for promoting significant neurological recovery.
  • Oligo(poly(ethylene glycol)fumarate) (OPF) hydrogel scaffolds have shown potential in supporting axonal regeneration.

Purpose of the Study:

  • To develop and evaluate a novel hybrid OPF scaffold with enhanced features for promoting axonal regeneration and functional recovery after SCI.
  • To investigate the impact of scaffold microarchitecture and extracellular matrix (ECM) protein coating on neuron attachment and axonal alignment.
  • To assess the ability of Schwann cells seeded on the scaffold to myelinate neurons.

Main Methods:

  • Fabrication of a hybrid OPF scaffold with a microarchitecture of polymer ridges that self-assemble into a spiral shape.
  • Coating the scaffold surface with ECM proteins (laminin, fibronectin, collagen I) to enhance cell attachment.
  • Seeding Schwann cells onto the scaffold and evaluating cell alignment, neurite outgrowth, and myelination of dorsal root ganglion neurons.

Main Results:

  • ECM protein coatings significantly promoted neuron attachment and axonal outgrowth on the scaffold.
  • The aligned microarchitecture of the scaffold effectively guided axons in a longitudinal orientation.
  • Closer ridge spacing increased cell and neurite alignment along the scaffold's direction.
  • Schwann cells seeded on the scaffold aligned with the ridges and successfully myelinated neurons over 4 weeks.

Conclusions:

  • The novel hybrid OPF scaffold with aligned microarchitecture and ECM coating represents a promising biomaterial construct for enhancing axonal regeneration after SCI.
  • This scaffold design facilitates directed axon growth and provides a supportive environment for Schwann cell-mediated myelination.
  • Further development of this scaffold holds potential for improving neurological recovery in patients with spinal cord injuries.