Evaluation of Maximum DNA Yield from a New Noninvasive Buccal Collection Device Following Various Extraction...

Area of Science:

• Forensic Science
• Molecular Biology
• Genetics

Background:

• Increasing demand for genetic testing necessitates efficient, non-invasive DNA collection methods.
• Buccal swabs offer a non-invasive alternative for deoxyribonucleic acid (DNA) recovery.
• High first-pass success rates in DNA testing depend on optimized collection and extraction protocols.

Purpose of the Study:

• To optimize two DNA extraction methods (PrepFiler™ Express Forensic Extraction and Maxwell® RSC Buccal Swab DNA Kits) for increased DNA yield from Gentueri's CollectEject™ Swab.
• To evaluate the impact of varying incubation parameters on DNA recovery efficiency.

Main Methods:

• Buccal swabs collected with Gentueri's CollectEject™ device were processed using forensic workflows.
• The PrepFiler method was optimized by adjusting incubation times and agitation intensity.
• The Maxwell method was optimized by varying incubation temperatures and durations.

Main Results:

• Increased agitation during PrepFiler extraction significantly improved DNA yield.
• Extended incubation times did not enhance DNA recovery with the PrepFiler method.
• No significant differences in DNA yield were observed for the Maxwell method across tested incubation temperatures and durations.

Conclusions:

• The CollectEject™ swab, combined with optimized extraction methods like PrepFiler, provides an efficient non-invasive approach for DNA collection.
• This method can consistently yield 5000 ng or more of DNA, meeting the demands of various genetic testing applications.
• Laboratories can integrate this simple buccal cell collection strategy into existing workflows for improved DNA recovery.