Related Experiment Video
Updated: Jul 16, 2026

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Published on: October 10, 2020
Association Between Glutathione S-Transferase (GST) Gene Polymorphisms and Coronary Artery Disease: A Case-Control
Sauda Sumaya Dina1, Somzida Akter Moni1, Faisal Abdullah Emon2
1Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka, Bangladesh.
Background:
Glutathione-S-transferases (GSTs) play an important role in the detoxification and neutralization of oxidative stress products and xenobiotic compounds. Due to genetic polymorphisms in GST genes, GSTs partially or completely lose enzymatic activity, which may increase susceptibility to cardiovascular diseases. The objective of this study was to investigate hitherto unknown GSTM1 and GSTT1 null genotype frequencies in patients with coronary artery disease (CAD) and their potential association with CAD in the Bangladeshi population.
Materials And Methods:
This study included 85 patients with CAD and 56 healthy control subjects confirmed by angiogram. Clinical parameters including triglyceride (TG), cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein levels were also evaluated. Genotypes of the GSTM1 and GSTT1 genes were identified using polymerase chain reaction followed by 1.5% (W/V) agarose gel electrophoresis with subsequent detection on a UV transilluminator.
Results:
There were significant differences in the null genotype distribution of GSTM1 and GSTT1 alleles between controls and patients with CAD. A significant association of the null genotype with CAD susceptibility (p < 0.05) was also observed. The combination of GSTM1 and GSTT1 null genes potentially increases the risk of CAD (odds ratio = 6.208; 95% confidence interval; p < 0.05). TG, LDL, and serum cholesterol levels were higher in patients with CAD (p < 0.05). GSTM1 and GSTT1 null genetic variations may be associated with increased susceptibility to CAD in the Bangladeshi population.
Conclusion:
These findings suggest a potential association between GST gene polymorphisms and CAD susceptibility; however, larger studies are required to provide more robust and generalizable evidence in the Bangladeshi population.
Related Concept Videos
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase
Pharmacogenomics: Identification of New Drug Targets
