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Updated: Nov 7, 2025
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Closed and High-Quality Bacterial Genome Sequences of the Oligo-Mouse-Microbiota Community
Quentin Lamy-Besnier1,2,3, Romain Koszul2, Laurent Debarbieux4
1Bacteriophage, Bacterium, Host Laboratory, Department of Microbiology, Institut Pasteur, Paris, France.
Microbiology Resource Announcements
|April 30, 2021
Abstract:
The Oligo-Mouse-Microbiota (OMM12) gnotobiotic murine model is an increasingly popular model in microbiota studies. However, following Illumina and PacBio sequencing, the genomes of the 12 strains could not be closed. Here, we used genomic chromosome conformation capture (Hi-C) data to reorganize, close, and improve the quality of these 12 genomes.
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