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Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
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Transcriptomic phases of periodontitis lesions using the nonhuman primate model.
Jeffrey L Ebersole1,2, Radhakrishnan Nagarajan3, Sreenatha Kirakodu4
1Department of Biomedical Sciences, School of Dental Medicine, B221, University of Nevada Las Vegas, 1001 Shadow Lane, Las Vegas, NV, 89106, USA. Jeffrey.ebersole@unlv.edu.
Scientific Reports
|April 30, 2021
Summary
This study reveals distinct gene expression patterns in primate gingival tissue during periodontitis initiation, progression, and resolution. Understanding these transcriptomic shifts aids in diagnosing and managing periodontal disease.
Area of Science:
- Periodontology
- Genomics
- Nonhuman Primate Models
Background:
- Periodontitis is a complex inflammatory disease affecting gum tissue.
- Understanding the molecular changes during disease progression and resolution is crucial for effective treatment.
Purpose of the Study:
- To identify gingival transcriptomic patterns during distinct phases of ligature-induced periodontitis in a nonhuman primate model.
- To delineate phase-specific gene expression profiles related to disease kinetics.
Main Methods:
- Ligature-induced periodontitis model in Macaca mulatta (primates).
- Gingival tissue sampling at baseline, during disease progression (2 weeks, 1, 3 months), and resolution (5 months).
- Gene expression analysis using microarray (Rhesus Gene 1.0 ST Array).
Main Results:
- Significant alterations in thousands of genes were observed during disease initiation and progression, predominantly up-regulated.
- Disease resolution showed altered gene expression, skewed towards down-regulation.
- Identified specific transcriptomic profiles related to epithelial, bone, immune, and inflammatory responses across disease stages.
Conclusions:
- Unique transcriptomic profiles characterize the exacerbation and remission phases of periodontitis.
- Phase-specific gene expression data can inform the development of biomarkers for human periodontal disease.
- Understanding these molecular dynamics may improve patient management strategies.
