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Published on: April 11, 2016
Examination of the usefulness of next-generation sequencing in mixed DNA samples
Fumi Momota1, Akiko Tsuji2, Atsushi Ishiko3
1Department of Forensic Pathology and Sciences, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan; Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, 912-9576, Japan.
Abstract:
The identification of individuals from mixed DNA samples is an important application of DNA typing. Although the discriminatory power of DNA profiling has improved dramatically, a limiting factor is that individuals cannot be identified via short tandem repeat (STR) analysis. We used next-generation sequencing (NGS) to examine the mixed DNA samples. Our results showed that STR nucleotide sequences and single nucleotide polymorphisms (SNPs) analysis via NGS may enable the identification of each distinct subject from a DNA mixture containing DNA of the victim and suspect.
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