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Recyclable laccase by coprecipitation with aciduric Cu-based MOFs for bisphenol A degradation in an aqueous
Yuhang Yuan1, Wenting Cai1, Jiaxin Xu1
1Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, College of Environment and Energy, South China University of Technology, Guangzhou, 510006, China.
Abstract:
Copper-based MOF (Cu-PABA) was selected to immobilize laccase (Lac) at optimum pH because of its favorable acid resistance. Cu-PABA@Lac biocomposites were synthesized in situ by the one-step method under moderate conditions (water environment and normal temperature and pressure). Cu-PABA@Lac had great potential to maintain stability due to the protection of the Cu-PABA shell and reasonable conformational changes. In addition, Cu-PABA@Lac could be used repeatedly by centrifugation, as confirmed in the degradation experiment of bisphenol A (BPA). Because of the synergistic effect of copper ions between laccase and Cu-PABA, the Km value decreased (from 0.0024 to 0.0014 mM); therefore, the affinity between laccase and guaiacol was enhanced. In conclusion, the system provides a choice for immobilized acid-resistant enzymes and a solution for environmental BPA degradation.
