Ratiometric fluorescence detection of anthrax biomarker based on terbium (III) functionalized graphitic carbon...

Detection of anthrax biomarker dipicolinic acid (DPA) is of great importance upon the crisis of bioterrorism. Development of fluorescent materials for DPA detection, particularly one that fully depends on single luminescent response, faces the challenge of being susceptible to interferences. The accompanying accuracy problems offer great opportunities for the establishment of more reliable ratiometric analysis method. Herein, a ratiometric fluorescent probe based on terbium functionalized graphitic carbon nitride nanosheets (Tb-g-C₃N₄NS) is attempted for quantitative detection of DPA to address the distinct function of g-C₃N₄NS as both carrier and reference fluorophore, which is a so-far unexplored option in fluorescent detection approaches. We achieve the incorporation of Tb³⁺ into framework of g-C₃N₄NS by using a simple synthetic strategy comprised of thermal pyrolysis and ultrasonic exfoliation. Combining the reference signal over g-C₃N₄NS at 440 nm (I₄₄₀) with the response signal of Tb³⁺ at 546 nm (I₅₄₆), concentration of DPA can be easily calculated via its linear correlation with the intensity ratio (I₅₄₆/I₄₄₀), giving a precise measurement towards DPA with a detection limit as low as 9.9 nM. Besides enabling an excellent self-calibrating detection of DPA, this work also inspires broader use of g-C₃N₄NS for relevant process.