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VecBase, a cloning vector sequence data base.

F Pfeiffer1, W A Gilbert

  • 1Martinsried Institut für Protein Sequence Data, Max-Planck-Institut für Biochemie, Federal Republic of Germany.

Protein Sequences & Data Analysis
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

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VecBase release 2 offers 98 cloning vectors, serving as a central repository. Caution is advised as sequences are assembled, not experimentally determined, necessitating careful documentation of discrepancies.

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • Cloning vectors are essential tools in molecular biology for DNA manipulation.
  • Existing databases may lack comprehensive or accurately assembled vector sequences.
  • VecBase aims to consolidate and document cloning vector sequences.

Purpose of the Study:

  • To establish VecBase as a central, reliable repository for cloning vector sequences.
  • To provide extensive documentation and flag potential sequence discrepancies.
  • To facilitate accurate use of cloning vectors in research.

Main Methods:

  • Compilation of 98 cloning vectors into 105 entries for VecBase release 2.
  • Inclusion of cross-references to parent sequences from VecBase and major DNA databases.

Related Experiment Videos

  • Detailed documentation highlighting sequence discrepancies and assembly methods.

    • Main Results:

    • VecBase release 2 contains 98 cloning vectors with extensive documentation.
    • Cross-references and discrepancy notes are provided for assembled sequences.
    • VecSource database added for ancillary sequences like promoters and polylinkers.

    Conclusions:

    • VecBase provides a valuable resource for molecular biologists needing accurate cloning vector information.
    • The emphasis on documentation and discrepancy flagging enhances the reliability of VecBase.
    • Continuous updates and expansion aim to make VecBase a comprehensive central repository.