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Updated: Nov 7, 2025
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of
Stefania Dell'Orso1, Aster H Juan2, Victoria Moiseeva3,4
1Genomic Technology Section, NIAMS, NIH, Bethesda, MD 20892, USA.
Abstract:
It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle stem cells from mouse tibialis anterior muscle by fluorescence-activated cell sorting and cDNA preparation. We also describe RNA extraction and cDNA preparation from differentiating mouse embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Juan et al. (2016) and Garcia-Prat et al. (2016).
