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Updated: Oct 18, 2026
Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
Published on: January 24, 2025
Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells
Ghazaleh Dadashizadeh1, Fadi Khalaf1, Stephanie Wojtowicz-Piotrowski2
1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Center for Burn Research, Hamilton Health Sciences, Hamilton, ON, Canada; Centre for Metabolism, Obesity, and Diabetes Research, McMaster University, Hamilton, ON L8S 4L8, Canada.
Abstract:
We present a protocol for isolating and culturing murine adipose progenitor cells (APCs) from adipose tissue. We describe steps for tissue dissection, unit-normalized enzymatic digestion, stromal vascular fraction (SVF) isolation, APC enrichment, and cell culture. We then detail quality control procedures that maximize cell yield, viability, and purity. This protocol combines enzymatic SVF isolation with magnetic-activated cell sorting enrichment to obtain a highly enriched APC population. It emphasizes reproducibility through unit-normalized digestion, controlled processing conditions, and standardized quality control metrics.
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