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Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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DNA Damage can Stall the Cell Cycle02:37

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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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DBF4, not DRF1, is the crucial regulator of CDC7 kinase at replication forks.

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Related Experiment Video

Updated: Nov 6, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

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Replication Stress, Genomic Instability, and Replication Timing: A Complex Relationship.

Lina-Marie Briu1, Chrystelle Maric1, Jean-Charles Cadoret1

  • 1Université de Paris, CNRS, Institut Jacques Monod, F-75006 Paris, France.

International Journal of Molecular Sciences
|May 5, 2021
PubMed
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Replication timing, crucial for genome organization, is linked to DNA replication stress and genomic instability. Understanding these connections may reveal new cancer therapies and biomarkers.

Keywords:
cancersgenomic instabilityreplication stressreplication timing

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Replication timing is vital for nuclear processes and genome organization in eukaryotes.
  • High-throughput studies link replication timing to transcription, epigenetics, mutation patterns, and 3D genome structure.
  • Replication stress, timing, and genomic instability are related, but their interplay is not fully understood.

Purpose of the Study:

  • To review the complex relationship between replication timing, replication stress, and genomic instability.
  • To highlight the impact of replication timing alterations in cancer cells.
  • To emphasize the need for further research into replication-timing regulation mechanisms.

Main Methods:

  • Literature review of recent high-throughput studies.
  • Analysis of existing evidence on replication timing, stress, and instability.
  • Discussion of molecular mechanisms and regulatory factors.

Main Results:

  • Replication timing is significantly altered in cancer cells, though causality is unclear.
  • A strong correlation exists between replication timing and epigenetic/mutational landscapes.
  • Replication stress is linked to genomic instability and replication timing alterations.

Conclusions:

  • Further research is needed to elucidate the molecular mechanisms regulating replication timing.
  • Identifying cis- and trans-acting factors is crucial for understanding replication timing.
  • Insights into replication timing could lead to novel therapeutic strategies and biomarkers for cancer and other diseases.