Morphological and biological properties of silica nanoparticles for CRTC3-siRNA delivery and downregulation of the...

The aim of this study was to characterize the morphological properties of amorphous silica nanoparticles (SiO₂ NPs), their cytotoxicity and intracellular location within Human Osteoblasts (HOB). Additionally, SiO₂ NPs were explored for their effectivity as carriers of CRTC3-siRNA on Human Preadipocytes (HPAd), and thus downregulate RGS2 gene expression. SiO₂ NPs were synthesized using the method of Stöber at 45 °C, 56 °C, and 62 °C. These were characterized via TEM with EDS, Zeta Potential and FT-IR. Cytotoxicity was evaluated by XTT at three concentrations 50, 100 and 500 µg/mL; SiO₂ NPs intracellular localization was observed through Confocal Laser Scanning Microscope. Delivering siRNA effectivity was measured by RT-qPCR. Morphology of SiO₂ NPs was spherical with a range size from 64 to 119 nm; their surface charge was negative. Confocal images demonstrated that SiO₂ NPs were located within cellular cytoplasm. At a SiO₂ NPs concentration of 500 µg/mL HOB viability decreased, while at 50 µg/mL and 100 µg/mL cell viability was not affected regardless SiO₂ NPs size. SiO₂ NPs-CRTC3-siRNA are effective to down-regulate RGS2 gene expression in HPAd without cytotoxic effects. The developed SiO₂ NPs-CRTC3-siRNA are a promising tool as a delivery vehicle to control obesity.