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Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes
Lilin Feng1, Liang Gao1, Daniel F Sauer1
1Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, Aachen 52074, Germany. u.schwaneberg@biotec.rwth-aachen.de.
Chemical Communications (Cambridge, England)
|May 5, 2021
Abstract:
We report a facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. A His6-tagged eGFP and four His6-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His6-tagged HRP.
