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Related Experiment Video

Updated: Nov 6, 2025

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma
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Published on: April 25, 2025

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Platelet Immunophenotyping by High-Dimensional Mass Cytometry.

Benjamin E J Spurgeon1, Alan D Michelson1, Andrew L Frelinger1

  • 1Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, Massachusetts.

Current Protocols
|May 5, 2021
PubMed
Summary
This summary is machine-generated.

Mass cytometry (CyTOF) enables detailed analysis of platelet surface markers, identifying distinct platelet subsets. This high-dimensional approach offers a deeper understanding of platelet roles in health and disease.

Keywords:
blood plateletscluster analysisflow cytometryimmunophenotypingplatelet activationplatelet function tests

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Area of Science:

  • Hematology
  • Immunology
  • Biotechnology

Background:

  • Platelets are crucial for hemostasis, immunity, and inflammation.
  • Characterizing platelet surface markers aids in diagnosing disorders and understanding platelet biology.
  • Conventional methods have limitations in comprehensively analyzing platelet phenotypes.

Purpose of the Study:

  • To describe the application of mass cytometry (CyTOF) for high-dimensional platelet immunophenotyping.
  • To demonstrate the identification of platelet subsets based on multi-marker expression.
  • To highlight the advantages of CyTOF over conventional flow cytometry for platelet analysis.

Main Methods:

  • Utilizing mass cytometry (CyTOF), which employs mass spectroscopy to detect metal-tagged antibodies on individual cells.
  • Measuring a large panel of markers on each platelet simultaneously.
  • Identifying platelet subsets by analyzing shared expression patterns of multiple markers.

Main Results:

  • CyTOF provides a significantly more detailed immunophenotype profile of platelets compared to traditional flow cytometry.
  • The method allows for the precise identification and characterization of distinct platelet subsets.
  • This enables a more granular investigation into platelet heterogeneity.

Conclusions:

  • Mass cytometry is a powerful tool for comprehensive platelet immunophenotyping.
  • The identification of platelet subsets using CyTOF can advance the understanding of their roles in various physiological and pathological conditions.
  • This high-dimensional approach opens new avenues for research in platelet biology and disease.