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Effect of GPIIb-IIIa complex ligands on calcium ion movement and cytoskeleton organization in activated platelets
F Sinigaglia1, A Bisio, M Torti
1Department of Biochemistry, University of Pavia, Italy.
Biochemical and Biophysical Research Communications
|July 15, 1988
Abstract:
We studied the influence of the occupancy of the fibrinogen receptor (GP IIb-IIIa complex) on two early aspects of agonist induced platelet activation: the increase of the intracellular Ca2+ concentration and the cytoskeleton reorganization. A monoclonal antibody, a peptide containing the RGD sequence and fibrinogen purified from human plasma were used as GP IIb-IIIa ligands. The obtained results demonstrated that fibrinogen receptor occupancy inhibits Ca2+ movement and cytoskeleton reorganization caused by low thrombin concentration and ADP.