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Updated: Nov 6, 2025

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
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The Phasor Plot: A Universal Circle to Advance Fluorescence Lifetime Analysis and Interpretation.

Leonel Malacrida1,2,3, Suman Ranjit1,4, David M Jameson5

  • 1Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92697, USA;

Annual Review of Biophysics
|May 6, 2021
PubMed
Summary

The phasor approach offers a unique, reproducible method for analyzing complex fluorescence signals in biological imaging. This technique aids in disease diagnostics by visualizing cellular states related to metabolism and oxidative stress.

Failed At:

2026-07-10T14:57:26.098181+00:00

Keywords:
FRETFörster resonance energy transferLAURDANNADHautofluorescencefluorescence lifetimephasor plots

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