Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Genomic Imprinting and Inheritance

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Predicting Recurrence Risk of Glioblastoma Based on Preoperative-Postoperative Longitudinal MRI: A Multicenter Study.

Bioengineering (Basel, Switzerland)·2026

Same author

Willingness to Undergo Corrective Surgery After Breast-Conserving Surgery in Chinese Patients with Breast Deformities: A Single-Center Study.

Breast cancer (Dove Medical Press)·2026

Same author

Investigation on capacitance variation under temperature and mechanical stress for the transfer capacitor in an electromagnetic pulse simulator.

The Review of scientific instruments·2026

Same author

Comprehensive outcomes of different prosthetic interfaces in total hip arthroplasty: A network meta-analysis and systematic review based on age and follow-up periods.

Medicine·2026

Same author

Functionalized metallic nanomaterials, nanozymes and nanomotors for emerging tumor diagnosis and treatment: from design to theranostics strategies.

Theranostics·2026

Same author

Lactylation in cardiac repair: Nexus and therapeutic opportunity.

Journal of molecular and cellular cardiology plus·2026

Same journal

Patient-derived organoids reveal ductal dysfunction and CFTR-modulator responses in chronic pancreatitis.

Cell stem cell·2026

Same journal

Lineage plasticity driven by GATA6 loss fuels colorectal cancer metastasis.

Cell stem cell·2026

Same journal

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation.

Cell stem cell·2026

Same journal

Paired pre- and post-transplant human immunoprofiling identifies an IFN-γ-JAK1 axis limiting stem-cell-derived RPE engraftment.

Cell stem cell·2026

Same journal

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026

Same journal

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 6, 2025

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Published on: February 24, 2023

Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing.

Rui Yan¹, Chan Gu¹, Di You¹

¹Center for Translational Medicine, Ministry of Education Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, China.

|May 6, 2021

Summary

This summary is machine-generated.

This study introduces scChaRM-seq, a new method to map DNA methylation, gene activity, and chromatin accessibility in single human oocytes. It reveals how these factors interact during oocyte development and gene regulation.

More Related Videos

Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Related Experiment Videos

Last Updated: Nov 6, 2025

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Published on: February 24, 2023

Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Area of Science:

Reproductive biology
Epigenetics
Genomics

Background:

Epigenetic reprogramming is crucial for female germ cell development.
Understanding DNA methylation dynamics in oocytes is limited.

Purpose of the Study:

To develop a single-cell multi-omics method for profiling DNA methylation, transcriptome, and chromatin accessibility.
To investigate de novo DNA methylation dynamics in human oocytes.

Main Methods:

Developed scChaRM-seq (single-cell Chromatin, RNA, and Methylome sequencing).
Simultaneously profiled DNA methylome, transcriptome, and chromatin accessibility in single human oocytes and ovarian somatic cells.

Main Results:

Observed global DNA methylation increase during human oocyte growth, correlating with chromatin accessibility.
Found specific DNA methylation increases linked to active transcription.
Identified species-specific gene expression and promoter accessibility influencing gene body methylation.
Alu elements remained hypomethylated and accessible near developmental genes.

Conclusions:

scChaRM-seq provides novel insights into DNA methylation pattern establishment during oocyte development.
Revealed interplay between DNA methylation, chromatin accessibility, and transcription in human oocytes.