Strategies for the valorisation of a protein-rich saline waste stream into polyhydroxyalkanoates (PHA)

Saline Mussels Cooking Wastewater was valorised to produce PHA with Mixed Microbial Cultures (MMC). Due to the high protein content (1.8-5.7 g COD_PROT/L), PHA accumulating capacity was below 10%, so several strategies were tested. In the acidification unit, Na(HCO₃) was added, increasing protein conversion into Volatile Fatty Acids (VFA) from 10.3% to 69.2% and subsequent PHA accumulation from 6.9 to 14.7%. In the enrichment unit, the incorporation of a settling stage after the feast phase provoked a shift in the proteins' oxidation from the feast to the famine phase, where the nitrogen released in the famine is used by the MMC for growth. This increased the biomass concentration and the tolerated COD (from 1.6 to 4.2 g VSS/L and from 2.2 to 4.38 g COD/L). Finally, varying the proteins/VFA ratio for MMC acclimation to proteins allowed increasing PHA accumulation from 8.8 to 41.5%.