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Updated: Nov 6, 2025

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
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Structures of the human Mediator and Mediator-bound preinitiation complex

Xizi Chen1, Xiaotong Yin1, Jiabei Li1

  • 1Fudan University Shanghai Cancer Center, Institutes of Biomedical Sciences, State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Radiation Oncology, and Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College of Fudan University, Shanghai 200032, China.

Science (New York, N.Y.)
|May 7, 2021
PubMed
Summary

Transcription factor IID (TFIID) orchestrates preinitiation complex assembly. The study reveals how TFIID-Mediator interactions stabilize RNA polymerase II C-terminal domain for phosphorylation, crucial for transcription initiation.

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