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Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering.

Lian He1, Peng Tan1,2, Lei Zhu3

  • 1Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX, USA.

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|May 7, 2021
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Summary

Researchers engineered new light-controlled protein switches (cpLOV2) for optogenetics. These versatile tools enable precise remote control of cellular functions and therapeutic applications, expanding possibilities in biotechnology.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Optogenetics

Background:

  • Plant-based photosensors, like light-oxygen-voltage sensing domain 2 (LOV2), offer modular control of cellular processes.
  • Current LOV2 applications are limited by caging surface options and effector domain placement.

Purpose of the Study:

  • To engineer novel LOV2 variants (cpLOV2) with enhanced caging capabilities.
  • To expand the toolkit of genetically encoded photoswitches for optogenetic applications.

Main Methods:

  • Circular permutation of the LOV2 domain.
  • Engineering of novel caging surfaces.
  • Demonstration of cpLOV2 in various optogenetic applications.

Main Results:

  • Developed cpLOV2 with expanded caging capabilities.
  • Successfully utilized cpLOV2 to control ion channels, CRISPR-Cas9, protein localization, and gene expression.
  • Generated photoactivatable CAR T cells for targeted tumor cell killing.

Conclusions:

  • cpLOV2 significantly enhances the versatility of optogenetic tools.
  • The engineered photoswitches are applicable to diverse biomedical and biotechnological engineering challenges.
  • This work accelerates the design of tailored optogenetic devices.