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Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors
Marites T Woon1, Puspa R Pandey1, Inbar Friedrich Ben-Nun2
1Cell and Gene Therapy Research and Development, Lonza Inc., Rockville, MD, USA.
Abstract:
Bioreactor technolology enables the expansion of mammalian cells, which can be translated to processes compatible with Current Good Manufacturing Practice (cGMP) regulations. Cells are introduced to the bioreactor vessel, wherein key parameters such as temperature, pH, and oxygen levels are tightly controlled to facilitate growth over time. Here, we describe the microcarrier-based expansion of human pluripotent stem cells in a 3 L stirred tank bioreactor.

