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Molecularly imprinted nanofilms for endotoxin detection using an surface plasmon resonance sensor.

Duygu Çimen1, Sevgi Aslıyüce1, Tuğçe Deniz Tanalp2

  • 1Hacettepe University, Department of Chemistry, Ankara, Turkey.

Analytical Biochemistry
|May 7, 2021
PubMed
Summary

This study developed a novel surface plasmon resonance (SPR) sensor for rapid and sensitive endotoxin detection using molecular imprinting. The sensor demonstrates high selectivity and a low limit of detection, validating its practical application.

Keywords:
EndotoxinMolecular imprintingSensorSurface plasmon resonance

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Endotoxins are potent pyrogens requiring sensitive detection methods.
  • Existing detection methods can be time-consuming or lack specificity.
  • Molecular imprinting offers a promising approach for creating selective biosensors.

Purpose of the Study:

  • To develop a simple, fast, sensitive, and selective surface plasmon resonance (SPR) sensor for endotoxin detection.
  • To utilize molecular imprinting for enhanced endotoxin recognition on SPR sensor surfaces.
  • To validate the sensor's performance against established methods.

Main Methods:

  • Synthesis of endotoxin-imprinted and non-imprinted nanofilms on SPR chips via UV polymerization.
  • Characterization of nanofilms using contact angle, atomic force microscopy, and ellipsometry.
  • Kinetic studies, selectivity tests, and validation using limulus amebocyte lysate (LAL) assay.

Main Results:

  • Achieved a low limit of detection (0.023 ng/mL) and quantification (0.078 ng/mL) for endotoxins.
  • Demonstrated a rapid response time of approximately 14 minutes for equilibration, adsorption, and regeneration.
  • Exhibited high selectivity for endotoxins over cholesterol and hemoglobin.

Conclusions:

  • The developed molecularly imprinted SPR sensor provides a simple, fast, and highly sensitive method for endotoxin detection.
  • The sensor's selectivity and rapid response time make it suitable for various applications.
  • Validation with LAL assay confirms the sensor's practical applicability and reliability.