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(±)-Gancochlearols J - N, renoprotective meroterpenoids from Ganoderma cochlear
Xiao-Hui Meng1, Fu-Ying Qin2, Xiao-Ting Jiang2
1School of Medicine, Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, People's Republic of China; School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, People's Republic of China.
Bioorganic Chemistry
|May 7, 2021
Summary
New meroterpenoids, gancochlearols J-N, were isolated from Ganoderma cochlear. Certain compounds showed potential in inhibiting kidney fibrosis, offering new therapeutic avenues.
Area of Science:
- Natural Product Chemistry
- Medicinal Chemistry
- Pharmacology
Background:
- Ganoderma species are a source of bioactive compounds.
- Meroterpenoids are complex natural products with diverse biological activities.
- Renal fibrosis is a significant pathological process in chronic kidney disease.
Purpose of the Study:
- To isolate and characterize new meroterpenoid enantiomers from Ganoderma cochlear.
- To determine the absolute configurations of the isolated compounds.
- To evaluate the potential of these compounds in inhibiting renal fibrosis.
Main Methods:
- Isolation and purification of compounds using chromatographic techniques.
- Structure elucidation using 1D and 2D Nuclear Magnetic Resonance (NMR) and High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS).
- Determination of absolute configurations via Electronic Circular Dichroism (ECD) calculations.
- In vitro biological evaluation using TGF-β1-induced rat kidney proximal tubular cells (NRK-52e).
Main Results:
- Five pairs of meroterpenoid enantiomers, designated as gancochlearols J-N (1-5), were successfully isolated.
- The structures and relative configurations of compounds 1-5 were determined.
- The absolute configurations of gancochlearols J-M (1-4) were assigned.
- (-)-Gancochlearol J ((-)-1) and (-)-Gancochlearol K ((-)-2) demonstrated inhibitory effects on renal fibrosis in a cellular model.
Conclusions:
- The study identified novel meroterpenoid enantiomers from Ganoderma cochlear.
- The findings suggest that (-)-gancochlearol J and (-)-gancochlearol K possess antifibrotic properties.
- These compounds represent potential leads for the development of therapeutic agents against renal fibrosis.
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