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Updated: Nov 6, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

DNA barcodes using a double nanopore system.

Swarnadeep Seth¹, Aniket Bhattacharya²

¹Department of Physics, University of Central Florida, Orlando, FL, 32816-2385, USA.

Scientific Reports

|May 8, 2021

Summary

This summary is machine-generated.

A double nanopore system can determine DNA barcodes, but chain dynamics cause measurement errors. Tension propagation influences DNA movement, necessitating advanced methods for accurate barcode deciphering.

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Last Updated: Nov 6, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

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Published on: October 31, 2013

Area of Science:

Biophysics
Nanotechnology
Genomics

Background:

Double nanopore systems offer potential for DNA barcode detection.
Accurate measurement of DNA segments between barcodes is crucial for applications like DNA sequencing.

Purpose of the Study:

To investigate the accuracy of DNA barcode length determination using a double nanopore system.
To identify the factors causing errors in length measurements and propose solutions.

Main Methods:

Brownian dynamics simulations of a coarse-grained DNA model with protein tags (barcodes).
Analysis of current blockade and time-of-flight data from simulations.
Development and validation of an interpolation scheme.

Main Results:

Variations in DNA segment velocities and tension propagation lead to under/overestimation of genetic lengths.
The motion of the entire DNA chain, governed by tension, explains non-uniform tag velocities.
An interpolation scheme using simulation data was introduced and validated for accurate barcode deciphering.

Conclusions:

Accurate DNA barcode measurement requires considering the dynamics of the entire DNA chain.
The proposed interpolation scheme can improve the accuracy of genetic length measurements in double nanopore systems.
Experimental implementation of the developed scheme is suggested for enhanced DNA barcode analysis.