Cotton DMP gene family: characterization, evolution, and expression profiles during development and stress
Shouhong Zhu1, Xinyu Wang2, Wei Chen1
1State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China.
Summary
This study identified 174 DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (DMP) genes in 16 plant species, revealing their roles in plant reproductive development and senescence. Cotton DMP genes are mainly expressed in reproductive organs and fiber, with potential applications in inducing haploids.
Area of Science:
- Plant molecular biology
- Genomics
- Biochemistry
Background:
- Members of the DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (DMP) gene family are plant-specific membrane proteins.
- DMPs are implicated in crucial physiological processes, including reproductive development and senescence.
Purpose of the Study:
- To conduct a genome-wide identification and analysis of DMP genes across 16 plant species.
- To investigate the phylogenetic relationships, duplication events, expression patterns, and potential functions of cotton DMP genes.
Main Methods:
- Phylogenetic analysis to classify DMP genes into subfamilies.
- Identification of duplicated gene pairs, particularly those arising from whole-genome duplication (WGD)/segmental duplications.
- Expression analysis in various cotton tissues and developmental stages.
- Analysis of cis-elements in GhDMPs promoter regions for abiotic stress response.
- Gene interaction network analysis to elucidate DMP involvement in biological pathways.
Main Results:
- A total of 174 DMP genes were identified, with 58 found in four cotton species, clustered into five subfamilies.
- 137 duplicated cotton gene pairs were identified, primarily resulting from WGD/segmental duplications.
- Cotton DMPs showed predominant expression in reproductive organs (sepal, petal, pistil, anther) and developing fiber.
- GhDMPs promoter regions exhibited differential responses to abiotic stress.
- DMPs are involved in plant senescence and flower reproductive development, with specific GhDMP/GbDMP genes proposed as candidates for inducing cotton haploids.
Conclusions:
- This study provides a comprehensive genome-wide analysis of the cotton DMP gene family.
- The findings offer insights into the potential functions of DMP genes in plant development, stress response, and reproductive processes.
- Specific DMP genes are identified as potential candidates for improving cotton breeding through haploid induction.
