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Oviductal Proteins Effect in Rooster Spermatic Cryopreservation
R Camarillo1, I Jimenez2, A Guzman3
1Mastery in the Animal Reproduction Biology, Metropolitan Autonomous University, San Rafael Atlixco, CDMX, Mexico.
Background:
Cryopreservation induces spermatic cryo capacitation, which can decrease thawed sperm fertilizing capability.
Objective:
To evaluate the effect of uterus-vaginal union protein factors to inhibit sperm cryo capacitation and maintain viability and fertilizing capability of rooster spermatozoa.
Materials And Methods:
Rooster spermatozoa was cryopreserved using Lake extender supplemented with different hen's uterus-vaginal junction protein concentrations, to determine spermatic viability, sperm physiological condition and fertilizing capability in vivo.
Results:
It was possible to induce spermatic decapacitation in vitro, inhibiting cryo capacitation and allowing fertility results comparable to those obtained with fresh semen.
Conclusion:
Uterus-vaginal protein extracts induce spermatic decapacitation in vitro.
