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Related Experiment Video

Updated: Nov 6, 2025

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07:03

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

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Piezo-ICSI for Human Oocytes.

Kenichiro Hiraoka1, Manami Isuge2, Yuko Kamada2

  • 1Kameda Medical Center; Kameda IVF Clinic Makuhari; Tokyo Medical and Dental University; ken.msn.hiraoka@gmail.com.

Journal of Visualized Experiments : Jove
|May 10, 2021
PubMed
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Piezo intracytoplasmic sperm injection (ICSI) offers higher survival and fertilization rates than conventional ICSI. This technique may improve assisted reproductive technology outcomes for human oocytes.

Area of Science:

  • Reproductive biology
  • Assisted reproductive technology (ART)

Background:

  • Intracytoplasmic sperm injection (ICSI) is a key ART technique.
  • Conventional ICSI involves micropipette aspiration, leading to low mouse oocyte survival (16%) and fertilization (8%) rates.
  • Piezo-ICSI, using a piezo drive and specialized micropipette, avoids cytoplasmic aspiration.

Purpose of the Study:

  • To describe a piezo-ICSI protocol.
  • To improve clinical results compared to conventional ICSI.
  • To assess the effectiveness of piezo-ICSI for human oocytes.

Main Methods:

  • Utilized a piezo drive unit and a flat-tipped micropipette for ICSI.
  • Implemented semi-automated membrane breakage without cytoplasmic aspiration.
  • Reviewed existing literature comparing piezo-ICSI and conventional ICSI for human oocytes.

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Last Updated: Nov 6, 2025

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Published on: July 23, 2011

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Human Egg Maturity Assessment and Its Clinical Application

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Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
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Main Results:

  • Piezo-ICSI demonstrated significantly higher survival (80%) and fertilization (78%) rates in mouse oocytes compared to conventional ICSI.
  • Five studies indicate piezo-ICSI yields significantly higher fertilization rates in human oocytes than conventional ICSI.

Conclusions:

  • Piezo-ICSI shows promise for enhancing human oocyte ICSI outcomes.
  • Further research is needed to fully establish piezo-ICSI's clinical effectiveness in humans.