CRISPR/Cas-Based Epigenome Editing: Advances, Applications, and Clinical Utility

Area of Science:

• Molecular Biology
• Genetics
• Epigenetics

Background:

• The epigenome regulates gene expression and cellular differentiation in eukaryotes.
• Epigenetic modifications are crucial for understanding biological processes and diseases.

Purpose of the Study:

• To review technical advancements in CRISPR/Cas-based epigenome editing.
• To examine the application of epigenome editing in human health and disease research.
• To discuss potential improvements for future utility of these technologies.

Main Methods:

• CRISPR/Cas-based epigenome editing technologies.
• Site-specific programming of epigenetic modifications.
• Manipulation of native chromatin architecture.

Main Results:

• Epigenome editing enables precise control over epigenetic marks and gene expression.
• These tools have elucidated causal links between epigenetic marks and gene expression.
• Applications in human health and disease research have expanded.

Conclusions:

• CRISPR/Cas epigenome editing is a powerful tool for studying epigenome function.
• Recent technical improvements have advanced clinical research.
• Further development will enhance the utility of epigenome editing for future applications.