Related Experiment Video
Updated: Nov 6, 2025

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells
Published on: June 30, 2023
ATG4: More Than a Protease?
Robin Ketteler1, Sharon A Tooze2
1MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
Abstract:
The ATG4 proteases are key regulators of autophagy. Until recently it was thought that their main function was to mediate the processing of ATG8 family members. A new study by Nguyen et al. reveals a role for ATG4s, independent of their catalytic activity, and proposes novel functions in mediating lipid transfer and mitophagy.
Related Concept Videos
Mechanical Protein Functions
GTPases and their Regulation
Large G-proteins,...
GTPases and their Regulation
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...

