Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.4K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.4K
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.5K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.5K
Patch Clamp01:18

Patch Clamp

5.9K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triphase Interfacial Nucleation Enables Microneedle-Templated Metal-Organic Framework Coating for <i>In Situ</i> DNA Capture and Release.

Analytical chemistry·2026
Same author

Long-term prognostic value of <sup>18</sup>F-FLT PET/CT versus <sup>18</sup>F-FDG PET/CT in locally advanced oesophageal squamous cell carcinoma: a prospective study.

Cancer imaging : the official publication of the International Cancer Imaging Society·2026
Same author

Regional variations of lipids in camel milk from Xinjiang, China: a UHPLC-MS/MS-based lipidomics study.

Frontiers in veterinary science·2026
Same author

Quantifying the differential climate responses of compound soil erosion by an integrated conceptual-machine learning framework.

Journal of environmental management·2026
Same author

Artificial MetalloDNAzymes with High-Density, Near-Atomic Precision Organization of Metal Cofactors for Enhanced Bioorthogonal Catalysis.

Journal of the American Chemical Society·2026
Same author

Dynamic Covalent Peptide-Drug Conjugates Address the Heterogeneity in Alzheimer's Disease Progression.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Bridging nanotechnology and mechanobiology.

Nature nanotechnology·2026
Same journal

Coherent 2D/3D van der Waals epitaxy enables single-crystal perovskite heterostructures.

Nature nanotechnology·2026
Same journal

Coherent 2D-3D van der Waals perovskite epitaxial heterostructures.

Nature nanotechnology·2026
Same journal

Ultrafast, reconfigurable all-optical beam steering and spatial light modulation.

Nature nanotechnology·2026
Same journal

A high-energy hydrogen radical initiates efficient electrosynthesis of urea from CO<sub>2</sub> and N<sub>2</sub>.

Nature nanotechnology·2026
Same journal

Machine-intelligent multimodal algebot for intracavitary chemotherapy.

Nature nanotechnology·2026
See all related articles

Related Experiment Video

Updated: Nov 6, 2025

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.8K

Bioorthogonal catalytic patch.

Zhaowei Chen1,2,3,4,5, Hongjun Li1,3,4,6, Yijie Bian7

  • 1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.

Nature Nanotechnology
|May 11, 2021
PubMed
Summary
This summary is machine-generated.

A novel microneedle patch delivers bioorthogonal catalysis for targeted cancer therapy. This device activates prodrugs locally, enhancing treatment efficacy and minimizing side effects in vivo.

More Related Videos

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

8.7K
The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits
10:15

The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

Published on: August 20, 2014

11.9K

Related Experiment Videos

Last Updated: Nov 6, 2025

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.8K
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

8.7K
The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits
10:15

The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

Published on: August 20, 2014

11.9K

Area of Science:

  • Artificial chemistry and bioorthogonal catalysis.
  • Nanomaterials and nanotechnology for biomedical applications.
  • Cancer therapy and drug delivery systems.

Background:

  • Transition metal-catalyzed bioorthogonal catalysis offers alternatives to enzymatic reactions for selective biomolecule labeling and in situ synthesis.
  • In vivo application of bioorthogonal catalysis is hindered by metal toxicity and complex administration methods.

Purpose of the Study:

  • To develop a safe and effective bioorthogonal catalytic device for in vivo applications.
  • To demonstrate the localized activation of prodrugs for enhanced cancer therapy with reduced side effects.

Main Methods:

  • Fabrication of a microneedle array patch integrating palladium (Pd) nanoparticles on titanium dioxide (TiO2) nanosheets.
  • In vivo testing of the patch for localized conversion of caged substrates to active forms.
  • Evaluation of prodrug activation at subcutaneous tumor sites and assessment of therapeutic anticancer effects.

Main Results:

  • The microneedle patch successfully mediated local conversion of caged substrates in vivo.
  • Subcutaneous application of the patch activated a prodrug at tumor sites, restoring its anticancer properties.
  • The localized treatment approach eliminated off-target prodrug activation and reduced systemic side effects.

Conclusions:

  • The developed bioorthogonal catalytic microneedle patch provides a robust and removable platform for localized in situ chemical transformations.
  • This technology offers a promising strategy for targeted cancer therapy, improving treatment efficacy and patient safety by minimizing off-target effects.