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Published on: January 28, 2018
Effect of Amide on Semen Cryopreservation of Curimba (Prochilodus lineatus)
I B Acosta1, C D Corcini2, S M M Gheller1
1Nucleus of Teaching and Research in Animal Reproduction, Federal University of Pelotas, Pelotas, Brazil.
Background:
The low molecular weight and high cellular permeability of amides make them suitable for use as penetrative cryoprotectants for sperm cells.
Objective:
This study aims to evaluate the effect of dimethylformamide (DMF) and dimethylacetamide (DMA) on sperm cryopreservation of Curimba (Prochilodus lineatus).
Materials And Methods:
Semen samples were diluted in media containing cryoprotectants [DMF, DMA and dimethyl sulfoxide (DMSO)]. Parameters of motility, membrane integrity, DNA integrity, mitochondrial functionality, viability and fertility were assessed upon thawing.
Results:
As compared to the 10% DMSO, DMA at 5% and DMF at 2% obtained the best results for the integrity of membrane, DNA and mitochondria; the motility parameters were best in the 2% and 5% DMF treatments. The best fertilization rates were demonstrated in 2%, 5%, and 8% DMF treatment groups.
Conclusion:
DMF at 2%, 5%, and 8% provided the best results for both in vitro and in vivo assessments, and can efficiently cryopreserve semen of Prochilodus lineatus.
