Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Organic fertilizer priming enhances eggplant seeds performance.

Brazilian journal of biology = Revista brasleira de biologia·2026
Same author

Genetic variability among guarana (Paullinia cupana var. sorbilis (Mart.) Ducke) provenances to support breeding strategies.

Brazilian journal of biology = Revista brasleira de biologia·2026
Same author

Optimizing semen cryopreservation in Calomys laucha: a step forward in rodent reproductive research.

Cryo letters·2024
Same author

Freezing of equine semen is influenced by exposure time and concentration of the cryoprotectant glycerol.

Cryo letters·2023
Same author

Trehalose in extenders for cryopreservation of Tambaqui (Colossoma macropomum) sperm.

Cryo letters·2023
Same author

In vivo hydrogen peroxide diffusivity in brain tissue supports volume signaling activity.

Redox biology·2022

Related Experiment Video

Updated: Nov 6, 2025

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.0K

Dimethylacetamide in Rooster Semen Cryopreservation.

N L S Gatti1, C D Corcini2, J S Filho3

  • 1Pos-Graduacao em Veterinaria, Faculdade de Veterinaria, Universidade Federal de Rio Grande, Rio Grande, Brasil.

Cryo Letters
|May 11, 2021
PubMed
Summary

Dimethylacetamide (DMA) at 6% for 3 minutes improved rooster semen cryopreservation. This concentration balanced protection and minimized sperm membrane damage, enhancing motility for potential commercial use.

More Related Videos

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

31.7K
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.2K

Related Experiment Videos

Last Updated: Nov 6, 2025

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.0K
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

31.7K
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.2K

Area of Science:

  • Animal Reproduction
  • Cryobiology
  • Sperm Biology

Background:

  • Sperm cryopreservation in cockerels presents significant challenges.
  • Limited information exists for commercial application of frozen rooster semen.

Purpose of the Study:

  • To evaluate the toxicity and efficacy of dimethylacetamide (DMA) as a cryoprotective agent for cockerel semen.
  • To determine optimal DMA concentration and equilibration time for sperm cryopreservation.

Main Methods:

  • DMA was tested at 3%, 6%, 9%, and 12% in freezing diluent.
  • Equilibration times varied from 1 to 9 minutes before freezing.
  • Post-thaw semen quality was assessed using CASA for kinetics and flow cytometry for membrane integrity and mitochondrial function.

Main Results:

  • 6% DMA with a 3-minute equilibration yielded the highest total (42.0%) and progressive (36.9%) motility.
  • This combination demonstrated an optimal balance between cryoprotective effect and plasma membrane integrity.

Conclusions:

  • A 6% DMA concentration with a 3-minute equilibration period is effective for cockerel sperm cryopreservation.
  • This method shows promise for improving the commercial viability of frozen rooster semen.